CRISPR at Lightning Speeds

Read more on this subject: Biology, Botany and Zoology
News Story Source:, by Brian Wang
They altered some of the guide RNA sequencing by adding light-sensitive nucleotides. Doing so prevented the guide from doing its job until light was applied. And once that light was applied, the binding took place within a matter of seconds. The researchers call it a "caged approach," because the guide is restrained until directed to carry out its job. They have named it very fast CRISPR (vfCRISPR).

Holding back the editing process and then activating it so quickly on-demand opens up the possibility of studying the process in more detail

This work uncovered many DNA repair pathways and formed the basis for the revolution in genome modification. That revolution in its most powerful manifestation came about with the discovery of RNA-directed DNA-cleaving enzymes in CRISPR systems, allowing the simple rules of base-pairing to guide targeted DNA breakage. Yet the CRISPR-associated 9 (Cas9) endonuclease that induces a DSB at target sites (determined by guide RNAs) in ge
Read More or Make a Comment

Bookmark the permalink.

Comments are closed.